[Putrescine--an inhibitor of the reaction of carbon dioxide hydration in hemolymph of lepidoptera].

Using high-voltage electrophoresis, thin-layer chromatography and chemical analysis tetramethylene diamine was found in hemolymph of 8 Lepidoptera species (Bombyx mori L., Ocneria dispar L., Euproctis chrysorrhoea L., Yponomeuta malinella L., Pieris brassicae L., Aporia crataegi L., Melacosoma neustria L., Mamestra brassicae L.). Its content reaches 11 mM 1 l of hemolymph. The absence of tetramethylene diamine in the intestine wall and Malpighian vessels and its presence in the fat body permits suggesting its synthesis in the fat body. Ion-selective H-electrodes were used to show the function of tetramethylene diamine as an inhibitor of the CO2 hydration reaction in the insects hemolymph.