黑腹果蝇中的β-羟基-α-酮丁酸，与果蝇蝶呤的生物合成相关

Beta-hydroxy-alpha-ketobutyric acid in Drosophila melanogaster, with reference to biosynthesis of drosopterins.

作者信息

Masada M, Akino M

出版信息

Biochim Biophys Acta. 1980 Jun 5;630(1):92-102. doi: 10.1016/0304-4165(80)90140-3.

Abstract

A substance designated as compound D, which reacts spontaneously with 7,8-dihydropterin to give drosopterins, is found in Drosophila melanogaster. The compound was partially purified from the extract of flies by column chromatography and identified as beta-hydroxy-alpha-ketobutyric acid by analysis of its 2,4-dinitrophenylhydrazone, mass spectrometry and reactivity with 7,8-dihydropterin. A highly significant correlation (r=0.969, p less than 0.001) was found between the amounts of the compound and drosopterins in the eye-pigment mutants of Drosophila. Changes of the compound during development of flies were also closely related to those of drosopterins. Based on these observations, a role of the compound in biosynthesis of drosopterins has been discussed.

摘要

在黑腹果蝇中发现了一种被命名为化合物D的物质，它能与7,8 - 二氢蝶呤自发反应生成果蝇蝶呤。该化合物通过柱色谱法从果蝇提取物中部分纯化，并通过对其2,4 - 二硝基苯腙的分析、质谱分析以及与7,8 - 二氢蝶呤的反应性鉴定为β - 羟基 - α - 酮丁酸。在果蝇眼色素突变体中，该化合物的含量与果蝇蝶呤的含量之间存在高度显著的相关性（r = 0.969，p < 0.001）。果蝇发育过程中该化合物的变化也与果蝇蝶呤的变化密切相关。基于这些观察结果，讨论了该化合物在果蝇蝶呤生物合成中的作用。