Szymanovicz A, Laurain-Guillaume G, Randoux A, Borel J P
C R Seances Acad Sci D. 1980 Mar 31;290(13):861-4.
A technique using alternatively dithiothreitol reduction, sonication and pepsin digestions permits the extraction of 75% of the insoluble collagen from human common iliac arteries. The pattern of the collagen types obtained by acrylamide gel electrophoresis is far different from that of the aortic wall: type I averages 44 +/- 14% of the total, type III 48.5 +/- 14% and a supplementary fraction, probably belonging to type V, 9 +/- 5% of the total in females and 6 +/- 2% in males.
一种交替使用二硫苏糖醇还原、超声处理和胃蛋白酶消化的技术能够从人髂总动脉中提取出75%的不溶性胶原蛋白。通过丙烯酰胺凝胶电泳获得的胶原蛋白类型模式与主动脉壁的模式有很大不同:I型胶原蛋白平均占总量的44±14%,III型占48.5±14%,还有一个补充部分,可能属于V型,在女性中占总量的9±5%,在男性中占6±2%。