吸入氯仿对肝脏葡萄糖醛酸化和去葡萄糖醛酸化机制的影响。

The effect of chloroform inhalation on hepatic glucuronidation and de-glucuronidation mechanisms.

作者信息

Capel I D, Jenner M, Dorrell H M, Williams D C

出版信息

Drug Chem Toxicol. 1980;3(1):73-81. doi: 10.3109/01480548009017834.

Abstract

The effect of 2, 4, 6 or 8 exposures to chloroform vapour on hepatic glucuronidating (UDPGA transferase) and de-glucuronidating (beta-glucuronidase) levels has been studied in rats. Successive treatments progressively decreased hepatic UDPGA transferase to a minimum of 53% of the control level. beta-Glucuronidase activity was increased two-fold after only two exposures and remained elevated for subsequent exposures. Cytochrome P450 levels decreased with each exposure. The level of this coenzyme in the treated animals remained lower than that of the control animals for at least 48 hours after treatment. UDPGA transferase was diminished to its lowest levels 9 hours after the final exposure to chloroform and did not achieve the control value for a further 48 hours. The beta-glucuronidase activity remained elevated for 12 hours after final exposure. The present experiment demonstrates that inhalation of toxic solvents such as chloroform decreases the glucuronidating capacity of the liver.

摘要

已对大鼠进行2次、4次、6次或8次氯仿蒸气暴露，研究其对肝脏葡萄糖醛酸化（UDPGA转移酶）和去葡萄糖醛酸化（β-葡萄糖醛酸酶）水平的影响。连续处理使肝脏UDPGA转移酶逐渐降低至对照水平的53%的最低值。仅两次暴露后，β-葡萄糖醛酸酶活性就增加了两倍，并且在随后的暴露中一直保持升高。每次暴露后细胞色素P450水平都会下降。在处理后至少48小时内，处理组动物中这种辅酶的水平仍低于对照组动物。最后一次暴露于氯仿9小时后，UDPGA转移酶降至最低水平，再过48小时才达到对照值。最后一次暴露后，β-葡萄糖醛酸酶活性持续升高12小时。本实验表明，吸入氯仿等有毒溶剂会降低肝脏的葡萄糖醛酸化能力。