胃泌素非硫酸化片段对猫胃酸和胃蛋白酶的刺激活性

Gastric acid and pepsin-stimulating activity of non-sulphated fragments of gastrin in the cat.

作者信息

Blair E L, Hirst B H, Lund P K, Reed J D, Sanders D J, Shaw B

出版信息

Digestion. 1980;20(3):190-200. doi: 10.1159/000198439.

DOI:10.1159/000198439

Abstract

The gastric acid and pepsin-stimulating activities of several C terminal fragments of non-sulphated human gastrin were investigated in the conscious cat prepared with cannulated gastric fistulae with a reproducible, continuous infusion method to construct dose-response curves. The gastric acid-stimulating activities were quantified by reference to the ED50 and were compared with the activity of synthetic non-sulphated human gastrin 17 (SHG17NS) and cat gastrin 17 (SCG17NS). SHG17NS had an ED50 of 0.329 nmol kg-1 h-1. SHG16NS, SHG15NS and SHG13NS all showed similar activities to SHG17NS. SCG17NS tested at low doses had similar activity to SHG17NS. SHG10NS, G5 and G4 and peptavlon showed progressively lower acid-stimulating activities: 26, 12, 9 and 9%, respectively, compared with SHG17NS. The gastric acid-stimulating activities of all the peptides were paralleled by similar pepsin-stimulating activities. The ratios of pepsin secretion:acid secretion were similar for all the peptides (1--2 microgram pepsin/microEq H+). The results are discussed in relation to the biological activity of the circulating gastrin components in the cat compared with other species and it is argued that component IV (corresponding to gastrin tetradecapeptide or minigastrin) is a major, physiologically active, circulating form of the hormone in the cat.

摘要

采用可重复的连续输注方法，通过胃瘘插管制备清醒猫，研究了几种非硫酸化人胃泌素C末端片段刺激胃酸和胃蛋白酶的活性，以构建剂量反应曲线。通过参考半数有效剂量（ED50）对胃酸刺激活性进行定量，并与合成的非硫酸化人胃泌素17（SHG17NS）和猫胃泌素17（SCG17NS）的活性进行比较。SHG17NS的ED50为0.329 nmol kg-1 h-1。SHG16NS、SHG15NS和SHG13NS均表现出与SHG17NS相似的活性。低剂量测试的SCG17NS与SHG17NS具有相似的活性。SHG10NS、G5和G4以及胃蛋白酶抑素的胃酸刺激活性逐渐降低：与SHG17NS相比，分别为26%、12%、9%和9%。所有肽的胃酸刺激活性均伴有相似的胃蛋白酶刺激活性。所有肽的胃蛋白酶分泌与胃酸分泌的比率相似（1-2微克胃蛋白酶/微当量H+）。结合猫与其他物种循环胃泌素成分的生物学活性对结果进行了讨论，并认为成分IV（对应于胃泌素十四肽或小胃泌素）是猫体内该激素主要的、具有生理活性的循环形式。