Tsukita S, Tsukita S, Ishikawa H
J Cell Biol. 1980 Jun;85(3):567-76. doi: 10.1083/jcb.85.3.567.
A filamentous network underlying the human erythrocyte membranes can be clearly visualized in situ by electron microscopy of thin sections of specimens fixed with tannic acid-glutaraldehyde. The network is composed of two layers: the first, a layer of vertical components with granular appearance, which are seen to be directly associated with the membrane proper, and the second, a horizontally disposed, anastomosing meshwork of filamentous components, approximately 9 nm in thickness, which are attached to the vertical components. The diameter and appearance of the filamentous components are similar to those of purified spectrin. EDTA treatment (0.1 mM, pH 8.0), which was used to extract spectrin and actin, resulted in the disappearance of the filamentous meshwork, leaving only the granular components.
通过对用鞣酸 - 戊二醛固定的标本薄切片进行电子显微镜观察，可以在原位清晰地看到人红细胞膜下的丝状网络。该网络由两层组成：第一层是具有颗粒外观的垂直成分层，可见其直接与细胞膜本身相关联；第二层是水平排列的、由丝状成分组成的吻合网络，厚度约为9纳米，附着在垂直成分上。丝状成分的直径和外观与纯化的血影蛋白相似。用于提取血影蛋白和肌动蛋白的EDTA处理（0.1 mM，pH 8.0）导致丝状网络消失，仅留下颗粒成分。
