Tanabe K, Kimata I, Takada S
J Parasitol. 1980 Apr;66(2):240-4.
The ability of Toxoplasma gondii tachyzoites to penetrate chick embryo erythrocytes (CEE) and the extent of penetration were examined in various simplified, incubation media. Toxoplasma gondii penetrated CEE well in serum-free Eagle's minimum essential medium or in Hanks' balanced salt solution, but much less in phosphate buffered saline (PBS). However, the addition of glucose alone to PBS substantially increased the penetration. A similar effect was seen with other monosaccharides, except galactose. A further, marked increase in penetration was noticed if magnesium ion (but not calcium ion) was added to PBS containing glucose. The present results indicate that T. gondii can penetrate CEE well in simplified incubation solutions such as PBS only when magnesium ion and a monosaccharide are added. Their possible role is discussed in relation to cellular adhesiveness, because the adherence of the parasite to the host cell membrane may be the first step in the penetration process.
在各种简化的孵育培养基中，检测了刚地弓形虫速殖子穿透鸡胚红细胞（CEE）的能力以及穿透程度。刚地弓形虫在无血清的伊格尔最低必需培养基或汉克斯平衡盐溶液中能很好地穿透CEE，但在磷酸盐缓冲盐水（PBS）中穿透能力则低得多。然而，仅向PBS中添加葡萄糖可显著提高穿透率。除半乳糖外，其他单糖也有类似效果。如果向含葡萄糖的PBS中添加镁离子（而非钙离子），则穿透率会进一步显著提高。目前的结果表明，只有添加镁离子和单糖时，刚地弓形虫才能在诸如PBS等简化的孵育溶液中很好地穿透CEE。鉴于寄生虫与宿主细胞膜的黏附可能是穿透过程的第一步，因此讨论了它们在细胞黏附方面可能发挥的作用。
