Cunningham D S, Kuhn R E
J Parasitol. 1980 Jun;66(3):390-8.
Spleen cells from normal mice and mice infected with Trypanosoma cruzi undergo mitogen-induced lymphoblast transformation in different ways. Some of these differences may be interpreted as suppression of the blastogenic response, whereas others cannot. Close examination of the culture conditions has enabled us to discriminate between in vivo-induced suppression and in vitro-determined (artifactual) suppression. Regardless of the type of suppression observed, it was shown to be dependent on duration of culture, length of labeling period, dose of mitogen, density of spleen cells, and method of expressing lymphoblast transformation data. The titration of mitomycin C-treated spleen cells from infected mice into cultures of normal spleen cells revealed two, separate, regulatory activities of spleen cells from infected mice as follow: (1) an enhancing effect which appeared late in infection and was obtained with low input of cells, and (2) a suppressive effect which appeared early in infection and was obtained with high input of cells. The enhancing effect was mediated by cells with properties characteristic of T-lymphocytes, whereas macrophages were found to be responsible for the suppressive effects.
正常小鼠和感染克氏锥虫的小鼠的脾细胞，以不同方式经历有丝分裂原诱导的淋巴细胞转化。其中一些差异可被解释为对增殖反应的抑制，而其他差异则不然。对培养条件的仔细检查使我们能够区分体内诱导的抑制和体外确定的（人为的）抑制。无论观察到的抑制类型如何，其都显示取决于培养持续时间、标记期长度、有丝分裂原剂量、脾细胞密度以及表达淋巴细胞转化数据的方法。将感染小鼠经丝裂霉素C处理的脾细胞滴定到正常脾细胞培养物中，揭示了感染小鼠脾细胞的两种独立调节活性，如下所示：（1）一种增强作用，出现在感染后期，且在低细胞输入量时获得；（2）一种抑制作用，出现在感染早期，且在高细胞输入量时获得。增强作用由具有T淋巴细胞特征特性的细胞介导，而发现巨噬细胞负责抑制作用。
