Cunningham D S, Kuhn R E
J Parasitol. 1980 Feb;66(1):16-27.
In vitro antisheep erythrocyte (SRBC) and antitrinitrophenyl (TNP) antibody responses of spleen cells obtained from C57BL/6 mice infected with Trypanosoma cruzi were reduced as early as 6 days postinfection and not detectable after 18 days of infection. Lymph node cells had normal antibody responses to SRBC and TNP in vitro until the 11th day of infection, after which responses were diminished. By day 31 of infection, lymph node cells were unresponsive to both SRBC and TNP in vitro. Not only were the antibody responses of spleen and lympho node cells to T-cell-dependent and -independent antigens progressively reduced as the period of infection increased, but in addition, the effect of lymphoid cell density and antigen dose on antibody production underwent several sequential changes. As the infection advanced, low densities of cultured lymphoid cells and low doses of antigen were ineffective in eliciting a detectable immune response, whereas high densities of lymphoid cells and high doses of antigen resulted in responses approximately equivalent to that observed with normal cells under the same conditions. Results of cell mixing studies have shown that a plastic-adherent, macrophage-like cell plays a major role in the suppressed humoral responses observed in this host-parasite system.
感染克氏锥虫的C57BL/6小鼠脾脏细胞的体外抗绵羊红细胞（SRBC）和抗三硝基苯（TNP）抗体反应早在感染后6天就降低了，感染18天后无法检测到。淋巴结细胞在感染后第11天之前对SRBC和TNP的体外抗体反应正常，之后反应减弱。到感染第31天时，淋巴结细胞在体外对SRBC和TNP均无反应。随着感染时间的延长，脾脏和淋巴结细胞对T细胞依赖性和非依赖性抗原的抗体反应不仅逐渐降低，而且淋巴细胞密度和抗原剂量对抗体产生的影响也经历了几个连续的变化。随着感染的进展，低密度的培养淋巴细胞和低剂量的抗原在引发可检测的免疫反应方面无效，而高密度的淋巴细胞和高剂量的抗原导致的反应与在相同条件下正常细胞观察到的反应大致相当。细胞混合研究结果表明，一种塑料贴壁的巨噬细胞样细胞在该宿主-寄生虫系统中观察到的体液反应抑制中起主要作用。
