[沼泽红假单胞菌中的硫代硫酸盐代谢]

[Thiosulfate metabolism in Rhodopseudomonas palustris].

作者信息

Rodova N A, Pedan L V

出版信息

Mikrobiologiia. 1980 Mar-Apr;49(2):221-6.

Abstract

The cells of the purple nonsulfur bacterium Rhodopseudomonas palustris, Nakamura strain, are capable of oxidizing thiosulfate and sulfide both under the anaerobic conditions in the light and under the aerobic conditions in the dark. Regardless of the presence of thiosulfate in the medium, the cells contain thiosulfate reductase, rodanase, thiosulfate oxidase, and sulfite oxidase. However, the capability to oxidize thiosulfate and sulfide is induced in Rh. palustris after the cells have been incubated in the presence of thiosulfate for 2--4 hours. The process of induction is related to the synthesis of protein components. Decomposition of thiosulfate in Rh. palustris when its concentration in the medium is low (2--5 mM) is accompanied with the formation of an equimolar quantity of sulfate. When the concentration of thiosulfate is higher (10--20 mM), the products of its oxidation are tetrathionate and sulfate. Therefore, the metabolic pathway of thiosulfate in Rh. palustris depends on its concentration in the medium.

摘要

紫色非硫细菌沼泽红假单胞菌中村菌株的细胞，在光照厌氧条件和黑暗需氧条件下均能氧化硫代硫酸盐和硫化物。无论培养基中是否存在硫代硫酸盐，细胞都含有硫代硫酸盐还原酶、硫氰酸酶、硫代硫酸盐氧化酶和亚硫酸盐氧化酶。然而，沼泽红假单胞菌在硫代硫酸盐存在下培养2至4小时后，氧化硫代硫酸盐和硫化物的能力才会被诱导。诱导过程与蛋白质成分的合成有关。当培养基中硫代硫酸盐浓度较低（2至5 mM）时，沼泽红假单胞菌中硫代硫酸盐的分解伴随着等摩尔量硫酸盐的形成。当硫代硫酸盐浓度较高（10至20 mM）时，其氧化产物为连四硫酸盐和硫酸盐。因此，沼泽红假单胞菌中硫代硫酸盐的代谢途径取决于其在培养基中的浓度。