MacColl R, Csatorday K, Berns D S, Traeger E
Biochemistry. 1980 Jun 10;19(12):2817-20. doi: 10.1021/bi00553a043.
Allophycocyanin, which is normally isolated as a trimer (alpha 3 beta 3), has now been successfully dissociated into a monomer (alpha beta) with strikingly different spectroscopic properties. In particular, upon dissociation the characteristic 650-nm absorption and 661-nm fluorescence emission bands of the trimer are completely lost and its fluorescence polarization properties are sharply altered. The spectroscopic characteristics of allophycocyanin monomers are much closer to those of C-phycocyanin than to trimeric allophycocyanin. A model for trimeric allophyocyanin is presented in which the appearance of the 650-nm absorption band is induced by a particular kind of chromophore-chromophore interaction. Similar results are found for both allophycocyanin II and III.
别藻蓝蛋白通常以三聚体（α3β3）形式分离得到，现在已成功解离为单体（αβ），其光谱性质有显著差异。特别是在解离时，三聚体特有的650纳米吸收带和661纳米荧光发射带完全消失，其荧光偏振特性也急剧改变。别藻蓝蛋白单体的光谱特征与C-藻蓝蛋白更为接近，而与三聚体别藻蓝蛋白相差较大。本文提出了一个三聚体别藻蓝蛋白的模型，其中650纳米吸收带的出现是由一种特定的发色团-发色团相互作用诱导的。别藻蓝蛋白II和III也得到了类似的结果。
