Yu C M, Brand J J
Biochim Biophys Acta. 1980 Jul 8;591(2):483-7. doi: 10.1016/0005-2728(80)90178-4.
Photosynthetic membrane fragments were prepared from Anacystic nidulans by French pressure cell disruption. Ascorbate was required to stabilize photophosphorylation activity in membranes kept at near 0 degrees C. Divalent cations were required during mechanical disruption and during assays for Photosystem II activity, with Ca2+ serving best. The rate of photophosphorylation was severely inhibited by Ca2+ during assays. Results suggest that best rates are achieved when photosynthetic membranes contain Ca2+ exposed to the interior surface, facilitating Photosystem II activity, and Mg2+ exposed to the exterior surface during assays, facilitating photophosphorylation activity.
通过法国压榨细胞破碎法从集胞藻中制备光合膜片段。在接近0摄氏度保存的膜中，需要抗坏血酸来稳定光合磷酸化活性。在机械破碎过程和光系统II活性测定过程中需要二价阳离子，其中Ca2+效果最佳。在测定过程中，Ca2+会严重抑制光合磷酸化速率。结果表明，当光合膜内表面暴露有Ca2+以促进光系统II活性，且在测定过程中外表面暴露有Mg2+以促进光合磷酸化活性时，可实现最佳速率。
