Mattox S M, Thompson G A
Biochim Biophys Acta. 1980 Jun 20;599(1):24-31. doi: 10.1016/0005-2736(80)90053-x.
Tetrahymena pyriformis cells have been grown in media varying in NaCl concentration from 3.7 mM (normal medium) to 0.3 M and varying in CaCl2 from 0.2 mM (normal medium) to 0.1 M. Tetrahymena grown in 0.3 M NaCl showed relatively few alterations in phospholipid composition, with significant changes being found only in the cell surface membranes (pellicle), which incrased in phosphatidylethanolamine content from 39% (low Na+) to 48% (high Na+) of the total phospholipids. The small decrease in fatty acid unsaturation and increase in shorter chain fatty acids in pellicle phospholipids were not statistically significant. No significant changes in phospholipid head group composition or fatty acid distribution were observed in high Ca2+-grown cells. Complementary studies of membrane fluidity, as inferred from freeze-fracture electron microscopy analysis, indicated that membranes of high Na+-acclimated cells were similar to those of control cells, when each was measured in its respective medium. However, the outer alveolar membrane of the pellicle and the food vacuolar membrane were considerably less fluid in high-Ca2+ cells. The lower fluidity in vacuolar membranes may have been responsible for alterations in the cells' capacity to form food vacuoles.
梨形四膜虫细胞在氯化钠浓度从3.7 mM（正常培养基）到0.3 M以及氯化钙浓度从0.2 mM（正常培养基）到0.1 M的不同培养基中培养。在0.3 M氯化钠中生长的梨形四膜虫在磷脂组成上显示出相对较少的变化，仅在细胞表面膜（表膜）中发现显著变化，其磷脂酰乙醇胺含量从总磷脂的39%（低钠）增加到48%（高钠）。表膜磷脂中脂肪酸不饱和度的小幅降低和较短链脂肪酸的增加在统计学上不显著。在高钙生长的细胞中未观察到磷脂头部基团组成或脂肪酸分布的显著变化。从冷冻断裂电子显微镜分析推断的膜流动性的补充研究表明，高钠适应细胞的膜与对照细胞的膜相似，前提是各自在其相应的培养基中进行测量。然而，在高钙细胞中，表膜的外泡膜和食物泡膜的流动性明显较低。液泡膜较低的流动性可能是细胞形成食物泡能力改变的原因。
