表明兔轻链可变区框架区和互补决定区独立排列的证据。一个可能的J小基因的描绘。
Evidence indicating independent assortment of framework and complementarity-determining segments of the variable regions of rabbit light chains. Delineation of a possible J minigene.
作者信息
Kabat E A, Wu T T, Bilofsky H
出版信息
J Exp Med. 1980 Jul 1;152(1):72-84. doi: 10.1084/jem.152.1.72.
Abstract
Amino acid sequences of rabbit light chains show considerable evidence of independent assortment of framework (FR) and complementarity-determining (CDR) segments. This suggests that they are coded for by independent genetic units (minigaenes) and that individual light chains are assembled somatically by recombining these units. Identical FR sets with multiple members generally comprise chains with different specificities, whereas identical CDR sets tend to have chains of a single specificity. A J segment, which, by analogy with mouse light chains, is made up of the last two residues of CDR3 plus all of FR4, contained 18 different sets and could contribute to diversity generated by CDR3. The longest segment, FR3, had a very large number of sets. Evidence is presented showing that the number of sets could be substantially reduced by permitting FR3 to be formed by two independently assorting segments comprising residues 57-68 and 69-88.
摘要
兔轻链的氨基酸序列显示出框架（FR）和互补决定（CDR）片段独立排列的大量证据。这表明它们由独立的遗传单位（微型基因）编码，并且个体轻链通过这些单位的重组在体细胞中组装。具有多个成员的相同FR集通常包含具有不同特异性的链，而相同的CDR集往往具有单一特异性的链。一个J片段，类似于小鼠轻链，由CDR3的最后两个残基加上所有的FR4组成，包含18个不同的集合，并且可能对CDR3产生的多样性有贡献。最长的片段FR3有非常多的集合。有证据表明，通过允许FR3由包含残基57 - 68和69 - 88的两个独立排列的片段形成，可以大幅减少集合的数量。
相似文献
1
Evidence indicating independent assortment of framework and complementarity-determining segments of the variable regions of rabbit light chains. Delineation of a possible J minigene.表明兔轻链可变区框架区和互补决定区独立排列的证据。一个可能的J小基因的描绘。
J Exp Med. 1980 Jul 1;152(1):72-84. doi: 10.1084/jem.152.1.72.
2
Evidence supporting somatic assembly of the DNA segments (minigenes), coding for the framework, and complementarity-determining segments of immunoglobulin variable regions.支持DNA片段（小基因）进行体细胞组装的证据，这些片段编码免疫球蛋白可变区的框架和互补决定区。
J Exp Med. 1979 Jun 1;149(6):1299-313. doi: 10.1084/jem.149.6.1299.
3
Variable region genes for the immunoglobulin framework are assembled from small segments of DNA--a hypothesis.免疫球蛋白框架的可变区基因是由小段DNA组装而成的——一种假说。
Proc Natl Acad Sci U S A. 1978 May;75(5):2429-33. doi: 10.1073/pnas.75.5.2429.
4
Structural evidence for independent joining region gene in immunoglobulin heavy chains from anti-galactan myeloma proteins and its potential role in generating diversity in complementarity-determining regions.来自抗半乳聚糖骨髓瘤蛋白的免疫球蛋白重链中独立连接区基因的结构证据及其在互补决定区产生多样性中的潜在作用。
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2890-4. doi: 10.1073/pnas.76.6.2890.
5
Identical V region amino acid sequences and segments of sequences in antibodies of different specificities. Relative contributions of VH and VL genes, minigenes, and complementarity-determining regions to binding of antibody-combining sites.不同特异性抗体中相同的V区氨基酸序列和序列片段。VH和VL基因、微型基因以及互补决定区对抗体结合位点结合的相对贡献。
J Immunol. 1991 Sep 1;147(5):1709-19.
6
kappa Chain joining segments and structural diversity of antibody combining sites.κ链连接片段与抗体结合位点的结构多样性
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4270-4. doi: 10.1073/pnas.77.7.4270.
7
Origins of antibody complementarity and specificity--hypervariable regions and minigene hypothesis.抗体互补性与特异性的起源——高变区与小基因假说
J Immunol. 1980 Sep;125(3):961-9.
8
Mechanisms of antibody diversity: multiple genes encode structurally related mouse kappa variable regions.抗体多样性的机制：多个基因编码结构相关的小鼠κ轻链可变区。
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3913-7. doi: 10.1073/pnas.75.8.3913.
9
Multiplicity of germline genes specifying a group of related mouse kappa chains with implications for the generation of immunoglobulin diversity.决定一组相关小鼠κ链的种系基因的多样性及其对免疫球蛋白多样性产生的影响。
Nature. 1978;276(5690):780-4. doi: 10.1038/276780a0.
10
Structural studies on induced antibodies with defined idiotypic specificities. IX. Framework differences in the heavy- and light-chain-variable regions of monoclonal anti-p-azophenylarsonate antibodies from A/J mice differing with respect to a cross-reactive idiotype.具有明确独特型特异性的诱导抗体的结构研究。IX. 来自A/J小鼠的单克隆抗对氨基苯砷酸盐抗体重链和轻链可变区在交叉反应独特型方面的框架差异。
J Exp Med. 1980 Apr 1;151(4):863-75. doi: 10.1084/jem.151.4.863.
引用本文的文献
1
Pathogenic relevance of IgG and IgM antibodies against desmoglein 3 in blister formation in pemphigus vulgaris.寻常型天疱疮水疱形成中桥粒芯糖蛋白 3 的 IgG 和 IgM 抗体的致病性相关性。
Am J Pathol. 2011 Aug;179(2):795-806. doi: 10.1016/j.ajpath.2011.04.015. Epub 2011 Jun 12.
2
Antigen-specific blocking of CD4-specific immunological synapse formation using BPI and current therapies for autoimmune diseases.利用 BPI 和当前的自身免疫性疾病疗法特异性阻断 CD4 特异性免疫突触的形成。
Med Res Rev. 2012 Jul;32(4):727-64. doi: 10.1002/med.20243. Epub 2011 Mar 23.
3
Fourteen nucleotides in the second complementarity-determining region of a human heavy-chain variable region gene are identical with a sequence in a human D minigene.人类重链可变区基因的第二个互补决定区中的14个核苷酸与人类D小基因中的一个序列相同。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5031-2. doi: 10.1073/pnas.79.16.5031.
4
cDNA clone encoding a complete rabbit immunoglobulin kappa light chain of b4 allotype.编码b4同种异型完整兔免疫球蛋白κ轻链的cDNA克隆。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4489-93. doi: 10.1073/pnas.80.14.4489.
5
Rearranged immunoglobulin heavy chain variable region (VH) pseudogene that deletes the second complementarity-determining region.重排的免疫球蛋白重链可变区（VH）假基因，其缺失第二个互补决定区。
Proc Natl Acad Sci U S A. 1984 Aug;81(16):5194-8. doi: 10.1073/pnas.81.16.5194.
本文引用的文献
1
Attempts to locate complementarity-determining residues in the variable positions of light and heavy chains.尝试在轻链和重链可变区定位互补决定残基。
Ann N Y Acad Sci. 1971 Dec 31;190:382-93. doi: 10.1111/j.1749-6632.1971.tb13550.x.
2
Binding of 2,4-dinitrophenyl compounds and other small molecules to a crystalline lambda-type Bence-Jones dimer.2,4-二硝基苯基化合物及其他小分子与一种结晶λ型本斯-琼斯二聚体的结合
Biochemistry. 1974 Aug 27;13(18):3816-27. doi: 10.1021/bi00715a031.
3
The structure determination of the variable portion of the Bence-Jones protein Au.本斯-琼斯蛋白Au可变部分的结构测定
Biophys Struct Mech. 1975 Feb 19;1(2):139-46. doi: 10.1007/BF00539775.
4
The molecular structure of a dimer composed of the variable portions of the Bence-Jones protein REI refined at 2.0-A resolution.由本斯-琼斯蛋白REI可变部分组成的二聚体的分子结构，分辨率为2.0埃。
Biochemistry. 1975 Nov 4;14(22):4943-52. doi: 10.1021/bi00693a025.
5
Immunoglobulin structures at high resolution.
Contemp Top Mol Immunol. 1975;4:127-53. doi: 10.1007/978-1-4615-8930-3_5.
6
Structural basis for the specificity of phosphorylcholine-binding immunoglobulins.磷酸胆碱结合免疫球蛋白特异性的结构基础。
Immunochemistry. 1976 Nov;13(11):945-9. doi: 10.1016/0019-2791(76)90239-1.
7
Rabbit antibody light chains: selective breeding narrows variability in framework and complementarity-determining residues.兔抗体轻链：选择性育种缩小了框架区和互补决定区残基的变异性。
Eur J Immunol. 1976 Aug;6(8):570-8. doi: 10.1002/eji.1830060809.
8
Amino acid sequence of rabbit light chains: variable region of a light chain from a homogeneous immunoglobulin raised by streptococcal immunization.兔轻链的氨基酸序列：由链球菌免疫产生的同源免疫球蛋白轻链的可变区。
Biochemistry. 1976 Apr 6;15(7):1381-6. doi: 10.1021/bi00652a005.
9
Preliminary refinement and structural analysis of the Fab fragment from human immunoglobulin new at 2.0 A resolution.
J Biol Chem. 1978 Jan 25;253(2):585-97.
10
Crystal structure of Bence Jones protein rhe (3 A) and its unique domain-domain association.
J Mol Biol. 1979 Apr 25;129(4):657-74. doi: 10.1016/0022-2836(79)90475-3.
Zotero 插件