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一种测定细胞膜跨膜电位的新方法。II. 无胆甾原体、支原体、链球菌和红细胞的膜电位

A novel method for the determination of electrical potentials across cellular membranes. II. Membrane potentials of Acholeplasmas, Mycoplasmas, Streptococci and erythrocytes.

作者信息

Schummer U, Schiefer H G, Gerhardt U

出版信息

Biochim Biophys Acta. 1980 Aug 14;600(3):998-106. doi: 10.1016/0005-2736(80)90502-7.

Abstract

The membrane potentials of Acholeplasma laidlawii, Mycoplasma mycoides subsp. capri, Mycoplasma gallisepticum, Streptococcus faecalis and human erythrocytes have been determined by applying a novel technique. The membrane potentials were calculated simply from potassium concentrations determined by atomic absorption spectroscopy, and gravimetry. The versatility of the new technique is demonstrated by comparing our results with data obtained by different techniques.

摘要

通过应用一种新技术测定了莱氏无胆甾原体、山羊支原体丝状亚种、鸡毒支原体、粪肠球菌和人红细胞的膜电位。膜电位仅根据通过原子吸收光谱法和重量法测定的钾浓度来计算。通过将我们的结果与通过不同技术获得的数据进行比较,证明了这项新技术的通用性。

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