实验性恙虫病中的宿主防御：定位控制小鼠天然抗性的基因。

Host defenses in experimental scrub typhus: mapping the gene that controls natural resistance in mice.

作者信息

Groves M G, Rosenstreich D L, Taylor B A, Osterman J V

出版信息

J Immunol. 1980 Sep;125(3):1395-9.

Abstract

Natural resistance of mice to lethal ifections of Rickettsia tsutsugamushi, strain Gilliam, is controlled by a single, autosomal, dominant gene, which we have designated Ric, with r and s representing the resistant nd susceptible alleles, respectively. Using three sets of recombinant inbred mouse strains (BXD, BXH, and BXJ), the Ric locus was mapped to Chromosome 5 closely linked to the retinal degeneration (rd) locus. This linkage was confirmed by a backcross analysis. Based on the RI strains and the C57BL/6Ty-le congenic strain (the only proven Ric-rd cross-over), we estimate the recombination frequency between Ric and rd to be 0.015. Three presumptive Ric-rd recombinants detected among 93 backcross mice may represent caes of incomplete penetrance of the resistance allele rather than recombination. Analyis of th C57BL/6JTy-le congenic strain indicates that Ric is proximal to rd on Chromosome 5. If so, the correct gene order is Pgm-1-W-Ric-rd-Gus.

摘要

小鼠对恙虫病东方体吉利亚姆株致死感染的天然抗性由一个常染色体显性单基因控制，我们将其命名为Ric，r和s分别代表抗性和易感等位基因。利用三组重组近交小鼠品系（BXD、BXH和BXJ），将Ric基因座定位到与视网膜变性（rd）基因座紧密连锁的5号染色体上。通过回交分析证实了这种连锁关系。基于重组近交系和C57BL/6Ty-le同源近交系（唯一经证实的Ric-rd交叉品系），我们估计Ric和rd之间的重组频率为0.015。在93只回交小鼠中检测到的三个推定的Ric-rd重组体可能代表抗性等位基因不完全外显的情况，而非重组。对C57BL/6JTy-le同源近交系的分析表明，Ric在5号染色体上位于rd近端。如果是这样，正确的基因顺序是Pgm-1-W-Ric-rd-Gus。