果蝇X病毒RNA聚合酶：双链病毒粒子RNA体外复制的初步模型。
Drosophila X virus RNA polymerase: tentative model for in vitro replication of the double-stranded virion RNA.
作者信息
Bernard J
出版信息
J Virol. 1980 Feb;33(2):717-23. doi: 10.1128/JVI.33.2.717-723.1980.
Abstract
An RNA polymerase activity was found to be associated with the infectious drosophila X virus particles extracted from infected flies. The rate of synthesis was at first linear as a function of time, and then a plateau was reached. During the linear phase of the synthesis, the template and product were associated as replicative intermediates which were larger than the double-stranded RNA of the drosophila X virus genome, but the final product of the reaction was indistinguishable from the RNA genome with respect to its density, sedimentation coefficient, electrophoretic mobility, and RNase resistance. The results indicated that both strands of the genomic RNA were copied. The implications of these findings with regard to other virion polymerases are discussed.
摘要
从受感染果蝇中提取的感染性果蝇X病毒颗粒与一种RNA聚合酶活性相关。合成速率起初与时间呈线性关系，随后达到平稳期。在合成的线性阶段，模板和产物作为复制中间体相互关联，这些中间体比果蝇X病毒基因组的双链RNA更大，但反应的最终产物在密度、沉降系数、电泳迁移率和对核糖核酸酶的抗性方面与RNA基因组无法区分。结果表明基因组RNA的两条链都被复制了。讨论了这些发现对其他病毒体聚合酶的意义。
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