Elsworth J D, Glover V, Sandler M
Psychopharmacology (Berl). 1980;69(3):287-90. doi: 10.1007/BF00433097.
Tele-methylhistamine, the first metabolite of histamine in tissues which lack diamine oxidase, is shown to be a substrate for human MAO B. Human liver homogenates were incubated with 3H-tele-methylhistamine and the products separated using thin-layer chromatography. The major product was 3-methylimidazoleacetic acid, the oxidatively deaminated metabolite of tele-methylhistamine. The reaction was inhibited by low concentrations of (-)deprenyl, the specific MAO B inhibitor. Tele-methylhistamine was also found to inhibit competitively the oxidation of phenylethlamine, but not that of 5-hydroxytryptamine, providing further evidence that it is oxidized by MAO B itself and not a related enzyme. This finding implies that (-)deprenyl and other MAO inhibitors used clinically may interfere with histamine metabolism.
在缺乏二胺氧化酶的组织中，组胺的首个代谢产物——tele-甲基组胺，被证明是人类单胺氧化酶B（MAO B）的底物。将人类肝脏匀浆与3H-tele-甲基组胺一起孵育，并用薄层色谱法分离产物。主要产物是3-甲基咪唑乙酸，即tele-甲基组胺的氧化脱氨基代谢产物。该反应受到低浓度的特异性MAO B抑制剂（-）司来吉兰的抑制。还发现tele-甲基组胺竞争性抑制苯乙胺的氧化，但不抑制5-羟色胺的氧化，这进一步证明它是由MAO B自身氧化，而非相关酶。这一发现意味着临床上使用的（-）司来吉兰和其他MAO抑制剂可能会干扰组胺代谢。
