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四种血清学检测方法在山羊布鲁氏菌病诊断中的比较

Comparison of four serological tests in the diagnosis of caprine brucellosis.

作者信息

Waghela S, Wandera J G, Wagner G G

出版信息

Res Vet Sci. 1980 Mar;28(2):168-71.

PMID:6774391
Abstract

Results from four serological tests for diagnosing brucellosis--serum tube agglutination (SAT), agar gel immunodiffusion (AGIT), Rose Bengal plate (RBPT) and complement fixation (CFT)--were compared using sera from goats from farms infected with Brucella melitensis. Ninety-two goats were negative and 29 positive to all four tests. The remaining 85 reacted to one or more tests. The RBPT was the most sensitive test and the AGIT the most specific, when compared with the CFT. The results suggest that the SAT adds little information when used with other tests but that RBPT and AGIT are useful for testing caprine brucellosis where facilities for the CFT are not available.

摘要

使用来自感染羊种布鲁氏菌农场的山羊血清,比较了四种用于诊断布鲁氏菌病的血清学检测方法——血清试管凝集试验(SAT)、琼脂凝胶免疫扩散试验(AGIT)、玫瑰红平板试验(RBPT)和补体结合试验(CFT)的结果。92只山羊对所有四项检测均呈阴性,29只呈阳性。其余85只对一项或多项检测有反应。与CFT相比,RBPT是最敏感的检测方法,AGIT是最特异的检测方法。结果表明,SAT与其他检测方法一起使用时提供的信息很少,但在没有CFT检测设施的情况下,RBPT和AGIT对于检测山羊布鲁氏菌病很有用。

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