Miller M, Kiermeier F, Weiss G, Klostermeyer H
Z Lebensm Unters Forsch. 1980 Jul;171(1):20-3. doi: 10.1007/BF01044412.
Usual TLC-methods for the determination of Aflatoxin M1 in milk and milk products sometimes do not separate interfering substances with similar optical and chemical properties from Aflatoxin M1, thus leading to considerably incorrect results. Even after an additional clean-up on polystyrene column the solutions contain so many compounds that the identification of Aflatoxin M1 is difficult. The resolving power of HPLC yield pure frations, which may be determined by their MS and UV spectra.
测定牛奶和奶制品中黄曲霉毒素M1的常用薄层色谱法有时无法将具有相似光学和化学性质的干扰物质与黄曲霉毒素M1分离,从而导致结果严重错误。即使在聚苯乙烯柱上进行额外的净化后,溶液中仍含有大量化合物,使得黄曲霉毒素M1的鉴定变得困难。高效液相色谱法的分离能力可产生纯馏分,这些馏分可通过其质谱和紫外光谱进行测定。