Dominguez L, Blanco J L, Gomez-Lucia E, Rodriguez E F, Suarez G
J Assoc Off Anal Chem. 1987 May-Jun;70(3):470-2.
A new method is described for the determination of aflatoxin M1 in milk and dairy products by thin layer chromatography. The main characteristic is the extraction system using an alkaline solution. Lipids are removed by centrifuging at low temperatures, and the aflatoxins are then extracted with CHCl3. The method has 2 options: Technique II (detection limit 0.02 ppb) requires cleanup on a chromatographic column; this is not necessary in Technique I (detection limit 0.1 ppb). The recovery rate in both techniques is over 92.8% in milk and yoghurt. This method may also be used for other aflatoxins. Because of the advantages of the method, Technique II is recommended for aflatoxin M1 control in milk, where a low detection limit is necessary. Technique I is proposed for experimental aflatoxin production studies in dairy products, which require analysis of a large number of samples but which do not require a very low detection limit.
本文描述了一种用薄层色谱法测定牛奶和乳制品中黄曲霉毒素M1的新方法。其主要特点是采用碱性溶液萃取系统。通过低温离心去除脂质，然后用CHCl₃萃取黄曲霉毒素。该方法有两种选择：技术II（检测限0.02 ppb）需要在色谱柱上进行净化；技术I（检测限0.1 ppb）则无需此步骤。两种技术在牛奶和酸奶中的回收率均超过92.8%。该方法也可用于其他黄曲霉毒素。由于该方法的优点，对于牛奶中黄曲霉毒素M1的控制，推荐使用技术II，因为需要低检测限。对于乳制品中黄曲霉毒素的实验性生产研究，建议使用技术I，此类研究需要分析大量样品，但不需要极低的检测限。
