Cocquempot M F, Garde V L, Thomas D
Biochimie. 1980;62(8-9):615-21. doi: 10.1016/s0300-9084(80)80108-8.
Lettuce thylakoïds were immobilized by the action of glutaraldehyde at subzero temperature in the presence of albumin. Foam structures with good mechanical properties were obtained. The activity yields for photosystem II and photosystems I + II were found equal to 71 per cent and 35 per cent respectively. The yield for ATP regeneration from ADP and Pi was 26 per cent. Increases of stability after immobilization were observed for all the functions of thylakoïds when stored and when continuously working. Spheroplasts and chromatophores from Rhodopseudomonas capsulata were immobilized with the same method; yields for ATP regeneration were found equal to 40 per cent and 70 per cent, respectively. An important increase of stability after immobilization was observed in both cases.
在白蛋白存在的情况下，通过戊二醛在零下温度的作用将生菜类囊体固定。获得了具有良好机械性能的泡沫结构。发现光系统II以及光系统I + II的活性产率分别等于71%和35%。由ADP和Pi再生ATP的产率为26%。当储存和连续工作时，类囊体的所有功能在固定后稳定性均有所提高。用相同方法固定荚膜红假单胞菌的原生质球和载色体；发现ATP再生的产率分别等于40%和70%。在这两种情况下均观察到固定后稳定性有显著提高。
