Shine T E, Little J R, Shore V, Medoff G, Abegg A
Eur J Immunol. 1980 Sep;10(9):714-8. doi: 10.1002/eji.1830100911.
Cholesterol and certain lipoproteins have regulatory effects on the primary immune responses of murine spleen cells in vitro. The plaque-forming cell (PFC) responses to sheep red blood cells of trinitrophenylated Brucella abortus were studied in complete, lipid-depleted or lipoprotein-reconstituted media. The requirement for exogenous low density lipoprotein (LDL) and its cholesterol moiety was established by comparison of the yield of PFC in cell cultures deprived of lipoproteins with that in cultures to which specific classes of lipoproteins were added. The spleen cells in complete medium yielded about 10-fold greater PFC responses than cells in lipoprotein-deficient medium. In lipoprotein-deficient media, human LDL completely reversed the decreased immune response, LDL lipids and free cholesterol partially reversed the deficit, the human high density lipoproteins and an apo B phospholipid complex were ineffective. In complete media, cholesterol at higher concentrations (100--200 microgram/ml) and LDL lipids partially inhibited the primary immune response. Exogenous cholesterol was required for the in vitro response to both thymus-dependent and thymus-independent antigens.
胆固醇和某些脂蛋白对小鼠脾细胞的体外初级免疫反应具有调节作用。在完全培养基、无脂培养基或脂蛋白重构培养基中研究了经三硝基苯化流产布鲁氏菌处理的脾细胞对绵羊红细胞的空斑形成细胞（PFC）反应。通过比较缺乏脂蛋白的细胞培养物与添加特定种类脂蛋白的培养物中PFC的产量，确定了对外源性低密度脂蛋白（LDL）及其胆固醇部分的需求。完全培养基中的脾细胞产生的PFC反应比脂蛋白缺乏培养基中的细胞高约10倍。在脂蛋白缺乏的培养基中，人LDL完全逆转了免疫反应的降低，LDL脂质和游离胆固醇部分逆转了缺陷，人高密度脂蛋白和载脂蛋白B磷脂复合物无效。在完全培养基中，较高浓度（100-200微克/毫升）的胆固醇和LDL脂质部分抑制了初级免疫反应。体外对胸腺依赖性和胸腺非依赖性抗原的反应都需要外源性胆固醇。
