Müller R, Babel W
Z Allg Mikrobiol. 1980;20(5):325-33. doi: 10.1002/jobm.3630200504.
Investigations of the 3-hexulosephosphate synthase (HPS) from different methylotrophic bacteria have revealed apparent discrepancies in kinetic behaviour. In all methanol-utilizing species investigated by us the kinetic characteristics showed intermediary plateau regions. Therefore, this behaviour is assumed to be a general feature of the HPS from all non-methane-utilizing methylotrophic bacteria. However, this assumption is in contrast to the results of other authors. Both for Methylomonas M15 (SAHM et al. 1976) and Methylomonas aminofaciens 77a (KATO et al. 1977, 1978) MICHAELIS-MENTEN kinetics of the HPS were stated. To check the validity of our assumption we have analyzed the kinetic data given by others. Indications of the existence of intermediary plateau regions could be found with the enzyme from Arthrobacter globiformis (BYKOVSKAYA and VORONKOV 1977) and Methylomonas aminofaciens 77a (KATO et al. 1978). Furthermore, biphasic ARRHENIUS plots indicate a multiple character of the HPS from these species as could already be demonstrated with the enzyme from Bacterium MB 58 and Pseudomonas oleovorans. In addition, causes which may obscure the detection of intermediary plateau regions are demonstrated.
对来自不同甲基营养型细菌的3-己酮糖磷酸合酶（HPS）的研究揭示了其动力学行为存在明显差异。在我们研究的所有利用甲醇的物种中，动力学特征显示出中间平台区域。因此，这种行为被认为是所有非利用甲烷的甲基营养型细菌中HPS的一个普遍特征。然而，这一假设与其他作者的结果相反。对于甲基单胞菌M15（SAHM等人，1976年）和氨基化甲基单胞菌77a（KATO等人，1977年、1978年），都阐述了HPS的米氏动力学。为了检验我们假设的有效性，我们分析了其他人给出的动力学数据。在球形节杆菌（BYKOVSKAYA和VORONKOV，1977年）和氨基化甲基单胞菌77a（KATO等人，1978年）的酶中可以发现存在中间平台区域的迹象。此外，双相阿累尼乌斯图表明这些物种的HPS具有多重特性，这在细菌MB 58和食油假单胞菌的酶中已经得到证实。此外，还证明了可能掩盖中间平台区域检测的原因。
