Pardo R, Larkin L H, Fields P A
Endocrinology. 1980 Dec;107(6):2110-2. doi: 10.1210/endo-107-6-2110.
Relaxin was localized in the cytoplasm of endometrial gland cells in the uterus of day 60 pregnant guinea pigs with the peroxidase-antiperoxidase method. The immunoprecipitate was located over the apical portions of the glandular cells. Stain was not found over non-endometrial portions of the uterus, endometrial surface epithelium or cells of the uterine cervical glands. Agar double-immunodiffusion analyses demonstrated a reaction of identity when extracts of uterus from day 60 pregnant guinea pigs and a porcine relaxin standard were tested with an antiserum produced against highly purified porcine relaxin. An extract of uteri from day 60 pregnant guinea pigs gave a positive response in the mouse uterus bioassay for relaxin.
采用过氧化物酶 - 抗过氧化物酶法，在妊娠60天的豚鼠子宫中，松弛素定位于子宫内膜腺细胞的细胞质中。免疫沉淀物位于腺细胞的顶端部分。在子宫的非子宫内膜部分、子宫内膜表面上皮或子宫颈腺细胞上未发现染色。琼脂双向免疫扩散分析表明，当用针对高度纯化的猪松弛素产生的抗血清检测妊娠60天的豚鼠子宫提取物和猪松弛素标准品时，出现了同一性反应。妊娠60天的豚鼠子宫提取物在小鼠子宫松弛素生物测定中呈阳性反应。
