Johnson M K, Boese-Marrazzo D
Infect Immun. 1980 Sep;29(3):1028-33. doi: 10.1128/iai.29.3.1028-1033.1980.
Of 12 strains of Pseudomonas aeruginosa, 10 were found to produce heat-stable extracellular hemolysin in highly aerated peptone broth supplemented with glycerol, fructose, or mannitol. Glucose supported good hemolysin production only in medium that was highly buffered. The yield of both cells and hemolysin was lower with organic acids as supplement. Growth-limiting phosphate concentrations produced maximum hemolysin levels. Purified hemolysin preparations contained two hemolytic glycolipids. The kinetics of hemolysis at various levels of purified lysin and the effects of variation in lysin and erythrocyte concentration are described.
在12株铜绿假单胞菌中，发现有10株在添加甘油、果糖或甘露醇的高度通气蛋白胨肉汤中产生热稳定的细胞外溶血素。葡萄糖仅在高度缓冲的培养基中能支持良好的溶血素产生。以有机酸作为补充剂时，细胞和溶血素的产量均较低。生长限制浓度的磷酸盐可产生最大溶血素水平。纯化的溶血素制剂含有两种溶血糖脂。本文描述了不同水平纯化溶血素时的溶血动力学以及溶血素和红细胞浓度变化的影响。
