Smith J K, Bowell P J, Bidwell E, Gunson H H
J Clin Pathol. 1980 Oct;33(10):954-7. doi: 10.1136/jcp.33.10.954.
Experimental evidence has been obtained that cryoprecipitation concentrates anti-A in mixtures of group O and group A plasma by a mechanism that does not operate in group O plasma alone. It has been concluded that the anti-A/A polysaccharide complex is less soluble during cryoprecipitation than anti-A immunoglobulin, and this complex dissociates to give free anti-A when the cryoprecipitate is redissolved. From a practical point of view, factor VIII concentrates prepared from cryoprecipitate obtained from single donations of plasma unselected for ABO group contain significantly less anti-A than those prepared from mixing pools of plasma in which partial neutralisation of anti-A has occurred before cryoprecipitation.
实验证据表明，在O型和A型血浆混合物中，冷沉淀通过一种仅在O型血浆中不起作用的机制浓缩抗A。已经得出结论，抗A/A多糖复合物在冷沉淀过程中的溶解度低于抗A免疫球蛋白，并且当冷沉淀重新溶解时，该复合物会解离产生游离的抗A。从实际角度来看，由未按ABO血型分组的单次血浆捐赠获得的冷沉淀制备的因子VIII浓缩物所含的抗A明显少于由在冷沉淀前已发生抗A部分中和的混合血浆池制备的因子VIII浓缩物。
