Sakaguchi N, Kishimoto T, Kikutani H, Watanabe T, Yoshida N, Shimizu A, Yamawaki-Kataoka Y, Honjo T, Yamamura Y
J Immunol. 1980 Dec;125(6):2654-9.
Stimulation of a murine pre-B cell line, 70Z/3, with LPS induced sIgM expression and an increase of L kappa-chain mRNA within 12 hr. In synchronized 70Z/3 cells, the cell division cycle was 10 to 11 hr and LSP-stimulation did not affect the cell division cycle. LPS-signals given at G1/S boundary induced sIgM expression in the G2 to M-phase. On the other hand, LPS-signals given after M-phase did not induce sIgM expression. Inhibition of cell division with demecorcin did not affect sIgM expression in the M-phase. Induction of intracellular kappa-chain synthesis was also observed in the G2 to M-phase when the LPS-stimulation was provided at G1/S boundary, but LPS-signals given after the M-phase did not induce de novo synthesis of kappa-chain. These results showed that LPS-induced sIgM expression and synthesis of kappa-chain were cell cycle-related events and sIgM expression was associated wih de novo synthesis of kappa-chain.
用脂多糖（LPS）刺激小鼠前B细胞系70Z/3，可在12小时内诱导表面免疫球蛋白M（sIgM）表达并使Lκ链信使核糖核酸（mRNA）增加。在同步化的70Z/3细胞中，细胞分裂周期为10至11小时，LPS刺激不影响细胞分裂周期。在G1/S边界给予的LPS信号在G2至M期诱导sIgM表达。另一方面，M期后给予的LPS信号不诱导sIgM表达。用去甲氧柔红霉素抑制细胞分裂不影响M期的sIgM表达。当在G1/S边界给予LPS刺激时，在G2至M期也观察到细胞内κ链合成的诱导，但M期后给予的LPS信号不诱导κ链的从头合成。这些结果表明，LPS诱导的sIgM表达和κ链合成是与细胞周期相关事件，且sIgM表达与κ链的从头合成相关。
