α-氯醇对恒河猴（猕猴属）附睾尾部精子葡萄糖代谢的影响。

Effect of alpha-chlorohydrin on glucose metabolism by spermatozoa from the cauda epididymidis of the rhesus monkey (Macaca mulatta).

作者信息

Ford W C, Harrison A

出版信息

J Reprod Fertil. 1980 Sep;60(1):59-64. doi: 10.1530/jrf.0.0600059.

DOI:10.1530/jrf.0.0600059

Abstract

Spermatozoa from the cauda epididymidis of the rhesus monkey had metabolic properties similar to those published for ejaculated spermatozoa. The concentration of glycolytic intermediates was low until 2 mM-glucose was added; glucose 6-phosphate, fructose 1,6-bis-phosphate and the triose plhosphates then increased but glycerate 3-phosphate did not. It was concluded that the activity of glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) limited flux through the glycolytic pathway. The production of lactate and CO2 from glucose was strongly inhibited in the presence of 5 or 10 mM-alpha-chlorohydrin. The energy charge of the spermatozoa was low before and after 1 incubation with 2 mM-glucose (0.5 +/- 0.05 and 0.05 +/- 0.06 respectively) and the value after the incubation was decreased in the presence of 5 or 10 mM-alpha-chlorohydrin (0.17 +/- 0.05 and 0.15 +/- 0.04 respectively). alpha-Chlorohydrin inhibited glycolysis at the glyceraldehyde 3-phosphate dehydrogenase reaction.

摘要

恒河猴附睾尾部的精子具有与已发表的射精精子相似的代谢特性。在添加2 mM葡萄糖之前，糖酵解中间产物的浓度较低；添加后，6-磷酸葡萄糖、1,6-二磷酸果糖和磷酸丙糖增加，但3-磷酸甘油酸没有增加。得出的结论是，3-磷酸甘油醛脱氢酶（EC 1.2.1.12）的活性限制了糖酵解途径的通量。在存在5或10 mM α-氯醇的情况下，葡萄糖产生乳酸和二氧化碳的过程受到强烈抑制。在与2 mM葡萄糖孵育前后，精子的能荷较低（分别为0.5±0.05和0.05±0.06），在存在5或10 mM α-氯醇的情况下，孵育后的能荷值降低（分别为0.17±0.05和0.15±0.04）。α-氯醇在3-磷酸甘油醛脱氢酶反应处抑制糖酵解。