Santidrián S, Young V R, Munro H N
Rev Esp Fisiol. 1980 Sep;36(3):255-63.
Male streptocozin diabetic rats were fed ad libitum in two diets, one a control, adequate in protein and energy, and another, depleted in protein, but adequate in energy. Within each one of these dietary groups, three hormone-treated groups were made as follows: rats receiving vehicle, or 0.25 or 0.50 I.U. insulin/100 g body weight/day i.p. for 21 days. A fourth group of intact rats, receiving vehicle injection, was included as a control. Every day urine excretion was collected for urea-N and 3-methylhistidine (3-Mehis) determination. Body weight and food intake were recorded daily. At the end of the experiment, all animals were sacrificed, and a sample of blood was taken for plasma insulin assay. Liver, as well as gastrocnemius, soleus and extensor digitorum longus muscles were excised and weighed. Results showed that diabetic animals had a reduced body weight gain, although the food intake was elevated in all groups, as compared to the intact rats. Gastrocnemius and soleus muscle weights were, respectively, reduced and increased in the diabetic animals fed the low-protein diet. Urea-N output was elevated in all groups fed the control diet, but a marked reduction was observed in the protein depleted rats. A reduction in 3-Mehis output was displayed by the diabetic animals, specially those fed the low-protein diet. The results of this experiment showed that in streptocozin diabetic rats there was a reduction in the rate of myofibrillar protein breakdown, specially marked when fed a protein depleted diet.
雄性链脲佐菌素糖尿病大鼠随意进食两种饮食，一种为对照饮食，蛋白质和能量充足，另一种蛋白质含量低，但能量充足。在每个饮食组中，按以下方式分为三个激素处理组：大鼠接受溶媒、或0.25或0.50国际单位胰岛素/100克体重/天腹腔注射，共21天。第四组为完整大鼠，接受溶媒注射作为对照。每天收集尿液用于测定尿素氮和3-甲基组氨酸（3-Mehis）。每天记录体重和食物摄入量。实验结束时，处死所有动物，采集血样进行血浆胰岛素测定。切除肝脏以及腓肠肌、比目鱼肌和趾长伸肌并称重。结果表明，糖尿病动物体重增加减少，尽管与完整大鼠相比，所有组的食物摄入量均升高。喂食低蛋白饮食的糖尿病动物，腓肠肌重量减少，比目鱼肌重量增加。喂食对照饮食的所有组尿素氮排出量均升高，但蛋白质缺乏的大鼠尿素氮排出量显著降低。糖尿病动物，特别是喂食低蛋白饮食的动物，3-Mehis排出量减少。本实验结果表明，链脲佐菌素糖尿病大鼠肌原纤维蛋白分解速率降低，喂食蛋白质缺乏饮食时尤为明显。
