Levieux D
Ann Rech Vet. 1980;11(1):89-97.
Samples of a single batch of milk or whey were subjected to various heat treatment and the rates of denaturation of whey proteins (beta-lactoglobulin and alpha-lactalbumin) evaluated by both immunological (radial immunodiffusion using specific antisera) and physical methods (G 100 filtration chromatography and polyacrylamide gel electrophoresis) in order to assess the utility and limitation of these techniques, and to clarify some conflicting resulted previously published. G 100 chromatography and radial immunodiffusion give very good results but the latter is very less time consumming and more quantitative. Polyacrylamide gel electrophoresis must be discarded for quantitative purpose since beta-lactoglobulin B peak is heavily contaminated by polymers of beta-lactoglobulin A, and alpha-lactalbumin peak by polymers of beta-lactoglobulin A and B.
对一批牛奶或乳清的样本进行了各种热处理，并通过免疫学方法（使用特异性抗血清的径向免疫扩散法）和物理方法（G 100过滤色谱法和聚丙烯酰胺凝胶电泳法）评估了乳清蛋白（β-乳球蛋白和α-乳白蛋白）的变性率，以便评估这些技术的实用性和局限性，并澄清先前发表的一些相互矛盾的结果。G 100色谱法和径向免疫扩散法给出了非常好的结果，但后者耗时更少且更具定量性。由于β-乳球蛋白B峰被β-乳球蛋白A的聚合物严重污染，α-乳白蛋白峰被β-乳球蛋白A和B的聚合物严重污染，因此出于定量目的必须舍弃聚丙烯酰胺凝胶电泳法。
