Etiévant M, Chevance L G, Lesourd M, Ohayon H
Ann Immunol (Paris). 1980 Jul-Aug;131D(1):13-42.
The action of activated complement on the upper respiratory tract was studied using the registration of ciliary beating and the transmission and scanning electron microscopy of ciliated cell lesions. The alternative pathway of activation was done using (1) non-specific activators, i. e. zymosan, dextran sulfate and polymerised sIgA, and (2) specific activators, i. e. "Ag-sIgA" immune complexes on normal mucosa and contact between an antigen and the tracheal mucosa of an immunized animal. In all cases, a quick alteration of the ciliary beating rythm was registered as well as more or less extensive cytological destruction. Such results were obtained with or without adjunction of complement; this suggested the existence of complement locally. A new method to demonstrate the occurrence of complement was developed, and the results showed that complement was present in the trachea. The above results were corroborated (1) by EDTA inhibition of the two complement pathways which prevented stopping of the ciliary beating and eliminated most of the cytological lesions, whilst EGTA left the alternative pathway operative with consequent alterations, and (2) by using C6-deficient rabbits in which no alterations were found. This study demonstrated that under certain conditions, local sIgA may activate the complement, and this fact explains many aspects of these immunological reactions of the respiratory epithelium.
利用纤毛摆动记录以及纤毛细胞损伤的透射电镜和扫描电镜观察，研究了活化补体在上呼吸道的作用。采用以下方法进行替代激活途径的研究：（1）非特异性激活剂，即酵母聚糖、硫酸葡聚糖和聚合sIgA；（2）特异性激活剂，即正常黏膜上的“抗原-sIgA”免疫复合物以及免疫动物的抗原与气管黏膜的接触。在所有情况下，均记录到纤毛摆动节律的快速改变以及或多或少广泛的细胞学破坏。无论是否添加补体，均得到此类结果；这提示局部存在补体。开发了一种新方法来证明补体的存在，结果显示气管中存在补体。上述结果得到了以下两方面的证实：（1）通过EDTA对两条补体途径的抑制作用，其可防止纤毛摆动停止并消除大部分细胞学损伤，而EGTA使替代途径仍起作用并导致相应改变；（2）通过使用C6缺陷兔，未发现任何改变。本研究表明，在某些条件下，局部sIgA可激活补体，这一事实解释了呼吸道上皮这些免疫反应的许多方面。
