异生物素与黑腹果蝇亚细胞组分的生物转化及光谱相互作用
Biotransformation and spectral interaction of xenobiotics with subcellular fractions from Drosophila melanogaster.
作者信息
Baars A J, Zijlstra J A, Jansen M, Vogel E, Breimer D D
出版信息
Arch Toxicol Suppl. 1980;4:54-8. doi: 10.1007/978-3-642-67729-8_11.
Several rapid bioassays are in use to detect, by means of mutagenicity, the formation of reactive metabolites from foreign compounds during their metabolism in the organism. Of these bioassays, the fruitfly Drosophila melanogaster was investigated with respect to its capacity to biotransform xenobiotics. By spectral analysis it was shown that in microsomal preparations of Drosophila the cytochromes P-450 and b5 are present. Microsomes appeared to possess aryl hydrocarbon hydroxylase and epoxide hydratase activities, while post-microsomal supernatants were able to conjugate appropriate compounds with glutathione and phosphate. As yet, glucosyl- and sulfotransferase activities can not be detected. The activities are compared to similar activities in rat liver.
目前有几种快速生物测定法可用于通过致突变性检测外来化合物在生物体内代谢过程中反应性代谢物的形成。在这些生物测定法中，对果蝇黑腹果蝇进行了研究，以了解其生物转化异源生物的能力。通过光谱分析表明，在果蝇的微粒体制剂中存在细胞色素P-450和b5。微粒体似乎具有芳烃羟化酶和环氧化物水合酶活性，而后微粒体上清液能够使适当的化合物与谷胱甘肽和磷酸盐结合。目前，尚未检测到葡糖基转移酶和磺基转移酶活性。将这些活性与大鼠肝脏中的类似活性进行了比较。
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