Conley M E, Cooper M D, Michael A F
J Clin Invest. 1980 Dec;66(6):1432-6. doi: 10.1172/JCI109998.
To further characterize the IgA deposits found in glomeruli of patients with IgA nephropathy, anaphylactoid purpura nephritis, and systemic lupus erythematosus, renal biopsies from patients with these disorders were stained by immunofluorescence with monoclonal anti-IgA subclass reagents, anti-light chain reagents and anti-J chain. The mesangium and peripheral capillary were brightly stained for IgA1 and were negative for IgA2. IgA1 and, to a lesser extent, IgA2 were contained in tubular casts. Both kappa and lambda light chains were found in all deposits. The intensity of J chain staining correlated with the intensity of IgM and not IgA staining. Biopsies brightly stained for IgA but negative for IgM were negative for J chain. These results indicate that glomerular IgA deposits in these disorders consist predominantly of monomers of IgA1.
为进一步明确在IgA肾病、过敏性紫癜性肾炎和系统性红斑狼疮患者肾小球中发现的IgA沉积物的特征，用单克隆抗IgA亚类试剂、抗轻链试剂和抗J链对这些疾病患者的肾活检组织进行免疫荧光染色。系膜和外周毛细血管IgA1染色呈强阳性，IgA2染色呈阴性。肾小管管型中含有IgA1，IgA2含量较少。所有沉积物中均发现κ和λ轻链。J链染色强度与IgM而非IgA染色强度相关。IgA染色呈强阳性但IgM染色呈阴性的活检组织J链染色呈阴性。这些结果表明，这些疾病中的肾小球IgA沉积物主要由IgA1单体组成。
