Janka Z, Szentistvanyi I, Rimanoczy A, Juhasz A
Psychopharmacology (Berl). 1980;71(2):159-63. doi: 10.1007/BF00434405.
The ionic regulating of lithium homeostasis and steady-state intra:extracellular lithium distribution in the brain can be approached by experimental methods using intact nerve cells in vitro. Primary cultures prepared from chick embryonic brain were applied to study the effect of extracellular sodium and potassium on the lithium uptake of nerve cells at 'therapeutic' lithium concentration (1.5 mM). Lithium influx and the level of steady-state intracellular lithium were significantly reduced by increasing the external sodium concentration. At physiological extracellular sodium level, the steady-state content of lithium in the brain cells was about half of that observed in the presence of 10 mM sodium in the incubation media and the value of the intra:extracellular lithium distribution ratio was below 1. External potassium (0.5 - 3mM) strongly inhibited lithium uptake of the nerve cells. Ouabain (10(-4)M) had no effect on this potassium-sensitive lithium uptake in Tyrode media. Sodium influx studied by isotope tracer methodology was higher in cultures preloaded with lithium as compared to that of the controls. It can be concluded that sodium and potassium ions, at physiological concentrations, significantly influence lithium uptake as well as the intra:extracellular lithium distribution in brain cell cultures.
细胞外锂的稳态分布可以通过体外使用完整神经细胞的实验方法来研究。应用从鸡胚脑制备的原代培养物来研究在“治疗性”锂浓度（1.5 mM）下细胞外钠和钾对神经细胞锂摄取的影响。通过增加细胞外钠浓度，锂流入量和细胞内锂的稳态水平显著降低。在生理细胞外钠水平下，脑细胞中锂的稳态含量约为在孵育培养基中存在10 mM钠时所观察到的一半，并且细胞内：细胞外锂分布比的值低于1。细胞外钾（0.5 - 3 mM）强烈抑制神经细胞的锂摄取。哇巴因（10⁻⁴ M）对Tyrode培养基中这种对钾敏感的锂摄取没有影响。通过同位素示踪法研究的钠流入量在预先加载锂的培养物中比对照组更高。可以得出结论，在生理浓度下，钠离子和钾离子显著影响脑细胞培养物中的锂摄取以及细胞内：细胞外锂分布。
