Duhm J, Eisenried F, Becker B F, Greil W
Pflugers Arch. 1976 Jul 30;364(2):147-55. doi: 10.1007/BF00585183.
Li+ net-transfer across cell membranes was studied on human erythrocytes and ghosts preloaded with 1-2 mM Li+ and incubated in saline media of varying composition at initial thermodynamic equilibrium for Li+. The following results were obtained: 1. Li+ is extruded from glycolyzing erythrocytes against an electrochemical gradient until a steady-state Li+ distribution is established after 24-28 h. 2. The initial rate of Li+ extrusion is not altered by ouabain or by reduction of ATP levels to less than 25% of the normal value. 3. Replacement of external Na+ by K+ or choline+ abolishes the establishment of an electrochemical Li+ gradient. 4. The Li+ distribution ratio Lie+/Lii+ increases proportional to the ratio Nae+/Nai+ at constant extravellular K+ concentrations. 5. In ghost suspension an uphill Li+ transport is driven by an oppositely directed Na+ gradient. The direction of the Li+ uphill transport can be reversed by reversing the Na+ gradient. From the results it is concluded that the Li+ uphill transport across human red cell membranes is mediated by a Na+-dependent Li+ counter-transport system. This system is not inhibited by ouabain and does not appear to be identical to the Na+-Na+ exchange system described by Garrahan and Glynn.
在预先加载了1-2 mM锂离子并在初始热力学平衡状态下于不同成分的盐溶液中孵育的人红细胞和血影上，研究了锂离子跨细胞膜的净转运。得到以下结果：1. 糖酵解红细胞中的锂离子逆电化学梯度被挤出，直到24-28小时后建立稳定状态的锂离子分布。2. 哇巴因或将ATP水平降低至正常值的25%以下，均不会改变锂离子挤出的初始速率。3. 用钾离子或胆碱离子替代外部钠离子会消除电化学锂离子梯度的建立。4. 在细胞外钾离子浓度恒定的情况下，锂离子分布比Lie+/Lii+与钠离子分布比Nae+/Nai+成正比增加。5. 在血影悬浮液中，上坡锂离子转运由相反方向的钠离子梯度驱动。通过反转钠离子梯度，可以反转锂离子上坡转运的方向。从这些结果可以得出结论，锂离子跨人红细胞膜的上坡转运是由一种依赖钠离子的锂离子反向转运系统介导的。该系统不受哇巴因抑制，似乎与Garrahan和Glynn描述的钠离子-钠离子交换系统不同。
