Peclaris G M
Endocrinol Exp. 1980 Dec;14(4):245-57.
Dispersed sheep adenohypophysial cells were used to study the effects of GnRH and ionic environment on LH, FSH and PRL release. The cells were used immediately after they were dissociated from the glands by a combination of gentle mechanical agitation and enzymatic digestion. GnRH caused a log-dose related release of LH and FSH with minimal and maximal effective doses of 10 pg ml-1 and 100 pg ml-1, respectively. Elevated level of K+ stimulated LH, FSH and PRL release. Maximal response was observed with the doses of 30--60 mmol l-1 K+ for LH and FSH, while a dose of 120 mmol l-1 was required for PRL release. The removal of Ca2+ by the addition of EDTA did not affect basal LH and FSH release but resulted in an increase of PRL release. It also abolished the release of LH and FSH induced by GnRH or by a high level of K+ but did not prevent a small and significant additive effect of K+ and GnRH. The release of PRL induced by a high level of K+ was inhibited by Ca2+ removal, but was not completely prevented at the highest K+ concentration used. The results demonstrated that the cells retained their responsiveness to secretagogues for LH, FSH and PRL immediately after dispersion.
采用分散的绵羊腺垂体细胞研究促性腺激素释放激素（GnRH）和离子环境对促黄体生成素（LH）、促卵泡生成素（FSH）和催乳素（PRL）释放的影响。细胞通过轻柔的机械搅拌和酶消化相结合的方法从腺体中解离出来后立即使用。GnRH引起LH和FSH呈对数剂量相关的释放，最小和最大有效剂量分别为10 pg/ml和100 pg/ml。升高的钾离子（K+）水平刺激LH、FSH和PRL的释放。对于LH和FSH，在30 - 60 mmol/L K+剂量时观察到最大反应，而PRL释放需要120 mmol/L的剂量。添加乙二胺四乙酸（EDTA）去除钙离子（Ca2+）不影响基础LH和FSH的释放，但导致PRL释放增加。它还消除了GnRH或高钾水平诱导的LH和FSH的释放，但不阻止钾离子和GnRH的小而显著的相加作用。高钾水平诱导的PRL释放被去除钙离子所抑制，但在所用的最高钾离子浓度下并未完全被阻止。结果表明，细胞在分散后立即保留了对LH、FSH和PRL促分泌素的反应性。
