High-performance liquid chromatography of acid-stable and acid-labile phosphoamino acids. - Suppr | 超能文献

High-performance liquid chromatography of acid-stable and acid-labile phosphoamino acids.

作者信息

Steiner A W, Helander E R, Fujitaki J M, Smith L S, Smith R A

出版信息

J Chromatogr. 1980 Dec 19;202(2):263-9. doi: 10.1016/s0021-9673(00)81735-5.

DOI:10.1016/s0021-9673(00)81735-5

Abstract

A high-performance liquid chromatographic system has been developed which permits the separation of both acid-stable and acid-labile phosphoamino acids. An anion-exchange resin and two buffers of different ionic strength and near neutral pH are used. A low-ionic-strength buffer is used for the separation of N-omega-phosphoarginine and N-epsilon-phospholysine, while the higher-ionic-strength buffer permits the clear separation of tau-phosphohistidine, omicron-phosphoserine and omicron-phosphothreonine. An in-stream fluorometric detection system using omicron-phthalaldehyde permits the rapid analysis of samples containing as little as 25 pmoles of phosphoamino acid. This method has been applied to the detection of tau-phosphohistidine from alkaline digests of chemically phosphorylated calf thymus histone 4 and bovine myelin basic protein.

摘要

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