Tholey G, Roth-Schechter B F, Mandel P
Neurochem Res. 1980 Aug;5(8):847-54. doi: 10.1007/BF00965784.
Primary cultures of glial cells prepared from brains of newborn rats were grown for periods of 1-5 weeks. After a proliferative phase of between 2 and 3 weeks, the cultures were maintained in stationary phase, during which a significant increase of oxygen consumption and of the activities of lactate dehydrogenase, succinate dehydrogenase, and mitochondrial glycerolphosphate dehydrogenase could be observed. Furthermore, qualitative changes in the lactate dehydrogenase isoenzyme pattern were found with time, characterized by a shift toward an enhanced synthesis of H subunits. A similar development was found in comparing the LDH isoenzyme pattern in the brain of 15-day-old rat embryo with those of newborn and adult rat brains. It is suggested that some aspects of maturation of glial cells in culture are comparable to those occurring in whole brain in vivo, namely a shift towards an enhanced aerobic metabolism.
从新生大鼠大脑制备的神经胶质细胞原代培养物培养1至5周。在2至3周的增殖期后，培养物维持在静止期，在此期间可观察到耗氧量以及乳酸脱氢酶、琥珀酸脱氢酶和线粒体甘油磷酸脱氢酶的活性显著增加。此外，随着时间的推移，发现乳酸脱氢酶同工酶模式发生了定性变化，其特征是向H亚基合成增强的方向转变。在比较15日龄大鼠胚胎大脑与新生和成年大鼠大脑的乳酸脱氢酶同工酶模式时发现了类似的发育情况。有人提出，培养的神经胶质细胞成熟的某些方面与体内全脑发生的情况相当，即向增强的有氧代谢转变。
