通过赖氨酸残基的可逆修饰实现蛋白质与核蛋白复合物的快速分离。
Ready separation of proteins from nucleoprotein complexes by reversible modification of lysine residues.
作者信息
Shetty J K, Kinsella J E
出版信息
Biochem J. 1980 Oct 1;191(1):269-72. doi: 10.1042/bj1910269.
Abstract
Modification of proteins with citraconic anhydride altered the electrostatic relationship between cationic epsilon-NH3+ groups of lysine residues of proteins and anionic phosphate groups of nucleic acids, thereby destabilizing the nucleoprotein complex. This procedure facilitated the separation of proteins from nucleic acids at pH4-4.2. The modifying groups were then deacylated from the proteins under acidic conditions (pH3-6) at 30 degrees C.
摘要
用柠康酸酐修饰蛋白质，改变了蛋白质赖氨酸残基的阳离子ε-NH3+基团与核酸阴离子磷酸基团之间的静电关系，从而破坏了核蛋白复合物的稳定性。此方法有助于在pH4 - 4.2条件下从核酸中分离蛋白质。然后在30℃的酸性条件（pH3 - 6）下，将修饰基团从蛋白质上去酰化。
相似文献
1
Ready separation of proteins from nucleoprotein complexes by reversible modification of lysine residues.
Biochem J. 1980 Oct 1;191(1):269-72. doi: 10.1042/bj1910269.
2
The reaction of citraconic anhydride with bovine alpha-crystallin lysine residues. Surface probing and dissociation-reassociation studies.
Biochim Biophys Acta. 1985 Apr 29;828(3):255-60. doi: 10.1016/0167-4838(85)90305-x.
3
Reversible modification of lysine in beta-lactoglobulin using citraconic anhydride. Effects on the sulfhydryl groups.
Int J Pept Protein Res. 1981 Jul;18(1):18-25. doi: 10.1111/j.1399-3011.1981.tb02035.x.
4
Lysinoalanine formation in yeast proteins isolated by alkaline methods.
J Agric Food Chem. 1980 Jul-Aug;28(4):798-800. doi: 10.1021/jf60230a019.
5
Conformational stability of citraconylated ovalbumin.
Int J Biochem. 1991;23(12):1375-84. doi: 10.1016/0020-711x(91)90278-u.
6
Stepwise modification of lysine residues of glucose oxidase with citraconic anhydride.
Int J Biol Macromol. 2006 Nov 15;39(4-5):192-6. doi: 10.1016/j.ijbiomac.2006.03.018. Epub 2006 Mar 28.
7
Reversible modification of lysine in soybean proteins, using citraconic anhydride: characterization of physical and chemical changes in soy protein isolate, the 7S globulin, and lipoxygenase.
J Agric Food Chem. 1980 Jul-Aug;28(4):818-24. doi: 10.1021/jf60230a016.
8
The action of thrombin on modified fibrinogen.
Thromb Haemost. 1983 Jun 28;49(3):208-13.
9
[Nucleoprotein complexes of yeast and their biological effect on T-lymphocytes].
Prikl Biokhim Mikrobiol. 2001 Jan-Feb;37(1):29-35.
10
The use of maleic anhydride for the reversible blocking of amino groups in polypeptide chains.
Biochem J. 1969 May;112(5):679-89. doi: 10.1042/bj1120679.
引用本文的文献
1
Using the Power of Organic Synthesis for Engineering the Interactions of Nanoparticles with Biological Systems.
Nano Today. 2016 Feb;11(1):31-40. doi: 10.1016/j.nantod.2015.11.002.
2
Polyelectrolyte Multilayers Fabricated from 'Charge-Shifting' Anionic Polymers: A New Approach to Controlled Film Disruption and the Release of Cationic Agents from Surfaces.
Soft Matter. 2008;4(8):1688-1695. doi: 10.1039/b804953j.
3
Characterization and in vitro methotrexate release from methotrexate/gelatin conjugates of opposite conjugate bond polarity.
Pharm Res. 2000 Oct;17(10):1309-15. doi: 10.1023/a:1026460023503.
4
Dicarboxylic acid anhydrides as dissociating agents of protein-containing structures.
Mol Cell Biochem. 1990 Sep 21;97(2):101-11. doi: 10.1007/BF00221051.
本文引用的文献
1
Tissue nucleic acids: 3. The nucleic acid and nucleotide content of liver tissue.
Biochem J. 1944;38(5):379-85. doi: 10.1042/bj0380379.
2
ALDOLASE DISSOCIATION INTO SUBUNITS BY REACTION WITH SUCCINIC ANHYDRIDE.
Biochemistry. 1964 Apr;3:535-41. doi: 10.1021/bi00892a012.
3
Reversible blocking of amino groups with citraconic anhydride.
Biochem J. 1968 Sep;109(2):312-4. doi: 10.1042/bj1090312.
4
Enzymic and immunochemical properties of lysozyme. Evaluation of several amino group reversible blocking reagents.
Biochemistry. 1970 Dec 8;9(25):4939-44. doi: 10.1021/bi00827a016.
5
Observations on the use of 2,4,6-trinitrobenzenesulphonic acid for the determination of available lysine in animal protein concentrates.
Analyst. 1973 Sep;98(170):673-86. doi: 10.1039/an9739800673.
6
Immunochemistry of sperm whale of myoglobin. IX. Specific interaction of peptides obtained by cleavage at arginine peptide bonds.
Biochemistry. 1971 May 11;10(10):1756-62. doi: 10.1021/bi00786a004.
7
Studies on the ribosome and its components.
Prog Nucleic Acid Res Mol Biol. 1976;17:77-98. doi: 10.1016/s0079-6603(08)60066-5.
8
Effect of succinylation on the oligomeric structure of arachin.
Int J Pept Protein Res. 1978 Apr;11(4):305-13. doi: 10.1111/j.1399-3011.1978.tb02854.x.
Zotero 插件