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酶技术的新可能性:固定化血红蛋白的羟化酶活性

[New possibility in enzymatic technology: hydroxylase activity of immobilized hemoglobin].

作者信息

Guillochon D, Ludot J M, Esclade L, Cambou B, Thomas D

出版信息

C R Seances Acad Sci III. 1981 Jan 26;292(4):365-8.

PMID:6781786
Abstract

Hydroxylation of Aniline by haemoglobin immobilized as cross-linked soluble polymers and insoluble particles was studied. Activity yields after immobilization as well as kinetic constants were estimated. Hydroxylase activities similar to liver microsomal cytochrome P450 activities were obtained.

摘要

研究了固定化为交联可溶性聚合物和不溶性颗粒的血红蛋白对苯胺的羟基化作用。估算了固定化后的活性产率以及动力学常数。获得了与肝微粒体细胞色素P450活性相似的羟化酶活性。

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