Bukhar M I, Vdovina N V, Krasnova L A, Koshcheenko K A
Prikl Biokhim Mikrobiol. 1980 Jul-Aug;16(4):503-10.
The effect of nonionogenic surface-active substances (SAS) on hydroxylase activity of Tieghemella orchidis in free and polyacrylamide gel (PAAG) immobilized states was studied. It was shown that addition of SAS (1%) to the cultivation medium simultaneously with the inoculation stimulated the filamentous growth of the mycelium. It was also demonstrated that certain SAS (span-80/sorbitanemonooleate/and polyethylene glycol-600-monolaurate) affected beneficially the culture transformation activity. The hydroxylase activity also increased after mycelium preincubation with tweens (1%) and transformation in the presence of span-20 (sorbitane monolaurate) and span-80 (0.3%). The use of the latter substances during gel preincubation with immobilized cells led to the stabilization of transformation activity, increase in the hydroxylation rate, and a change in the yield of hydroxylated products to produce hydrocortisone.
研究了非离子表面活性物质（SAS）对处于游离态和聚丙烯酰胺凝胶（PAAG）固定态的兰花铁刀木羟化酶活性的影响。结果表明，在接种的同时向培养基中添加1%的SAS会刺激菌丝体的丝状生长。还证明了某些SAS（司盘-80/脱水山梨醇单油酸酯/和聚乙二醇-600-单月桂酸酯）对培养物转化活性有有益影响。用吐温（1%）对菌丝体进行预培养，并在司盘-20（脱水山梨醇单月桂酸酯）和司盘-80（0.3%）存在的情况下进行转化后，羟化酶活性也有所增加。在凝胶与固定化细胞预培养过程中使用后一种物质，导致转化活性稳定、羟化速率提高以及羟化产物产量发生变化，从而产生氢化可的松。
