Sewell D L, Bryant R E, Rashad A L, Miller M J
Ann Clin Lab Sci. 1981 Jan-Feb;11(1):15-8.
Fluorescein isothiocyanate-labelled goat antihuman immunoglobulin (anti-IgG, anti-IgA, anti-IgM) and anticomplement conjugates were used to detect antibody or complement-coated bacteria from the oropharynx of patients with pharyngitis. Throat smears prepared from patients with a positive culture for group A beta-hemolytic streptococci had significantly more bacteria that stained with labelled antihuman IgG than smears prepared from patients with a negative culture. When compared to the results of a throat culture, the sensitivity, specificity, and predictive value of a positive and negative antihuman IgG stained smear was 91 percent, 94 percent, 85 percent, and 96 percent, respectively. The results of smears stained with antihuman IgA, IgM or complement did not correlate statistically with the isolation of group A beta-hemolytic streptococci.
异硫氰酸荧光素标记的山羊抗人免疫球蛋白（抗IgG、抗IgA、抗IgM）和抗补体结合物用于检测咽炎患者口咽部抗体或补体包被的细菌。A组β溶血性链球菌培养阳性患者制备的咽涂片，与培养阴性患者制备的涂片相比，用标记的抗人IgG染色的细菌明显更多。与咽培养结果相比，抗人IgG染色涂片阳性和阴性的敏感性、特异性及预测值分别为91%、94%、85%和96%。用抗人IgA、IgM或补体染色的涂片结果与A组β溶血性链球菌的分离无统计学相关性。
