Satoh H, Mito Y, Ueda T, Kurihara K, Kobatake Y
Biochim Biophys Acta. 1980 Dec 15;633(3):436-43. doi: 10.1016/0304-4165(80)90201-9.
The plasmodium of Physarum polycephalum was treated with Ca2+ ionophores A23187 or X-537A, and the effects on the chemotactic and contractile activities were studied by applying a double-chamber method and an optical method. (1) At low Ca2+ concentration, attractants (glucose, L-alanine) KH2PO4) remained as attractants and induced a relaxation in the plasmodial strand. Increase in the external Ca2+ level caused a contraction of plasmodium with the same stimuli, and hence converted the attractants to repellents. (2) This phenomenon was specific to Ca2+; Mg2+, Sr2+ or Ba2+ showed no appreciable effects. (3) Repellents (sucrose, KCl, Quinine) induced a contraction, which was not altered by the Ca2+ ionophore treatment. (4) Efflux of Ca2+, which was measured by chemiluminescence of aequorin, increased on glucose reception in the presence of ionophore.
用钙离子载体A23187或X - 537A处理多头绒泡菌的原质团，并通过双室法和光学方法研究其对趋化活性和收缩活性的影响。（1）在低钙离子浓度下，引诱剂（葡萄糖、L - 丙氨酸、KH2PO4）仍作为引诱剂，并诱导原质团丝松弛。外部钙离子水平的升高会使原质团在相同刺激下收缩，从而将引诱剂转变为驱避剂。（2）这种现象对钙离子具有特异性；镁离子、锶离子或钡离子未显示出明显影响。（3）驱避剂（蔗糖、氯化钾、奎宁）诱导收缩，钙离子载体处理未改变这种收缩。（4）在离子载体存在的情况下，通过水母发光蛋白的化学发光测量的钙离子外流在葡萄糖接收时增加。
