A method for the purification of bovine vitreous body hyaluronic acid.

Bovine vitreous body hyaluronic acid was treated with activated CH-Sepharose 4B after purification with cold 5% trichloroacetic acid. By this method a highly purified hyaluronic acid can be prepared. Contaminating serum proteins were completely removed. The remaining small amounts (0.3%) of the protein contaminants were found, by immunological methods, to be non-plasma proteins.