Enfors S O, Molin G
Can J Microbiol. 1981 Jan;27(1):15-9. doi: 10.1139/m81-003.
The growth inhibitory effect of 50 kPa (0.5 atm) CO2 was tested for Pseudomonas fragi in the temperature range 5-35 degrees C and of 101 kPa (1 atm) CO2 on Bacillus cereus in the range 18-46 degrees C. The maximum specific growth rate (mumax) of P. fragi in air (pH 6.7) was 0.44 h-1 at 35C, 0.66 h-1 at 30 degrees C, and 0.078 h-1 at 5 degrees C. In 50 kPa of CO2 in air the relative inhibition of the growth rate was about 30% at 35 degrees C, 50% at 30 degrees C, and 90% at 5 degrees C. Thus, the inhibitory effect of CO2 successively increased with decreasing temperature, an effect which was explained by the increasing solubility of CO2 with decreasing temperature. The anaerobic growth of B. cereus (101 kPa N2) was optimal at 40 degrees C and stopped at temperatures below 18 degrees C and above 46 degrees C. The relative inhibitory effect of 101 kPa CO2 at the optimum growth temperature was about 40%; this increased to 100% near the maximum and minimum growth temperatures. The growth inhibitory effect of reduced temperature (below optimum) and CO2 and B. cereus was larger than that expected from the increased solubility of CO2 at lower temperatures.
测试了50 kPa（0.5个大气压）二氧化碳在5至35摄氏度温度范围内对脆弱拟杆菌的生长抑制作用，以及101 kPa（1个大气压）二氧化碳在18至46摄氏度范围内对蜡样芽孢杆菌的生长抑制作用。脆弱拟杆菌在空气中（pH 6.7）的最大比生长速率（μm ax）在35摄氏度时为0.44 h⁻¹，在30摄氏度时为0.66 h⁻¹，在5摄氏度时为0.078 h⁻¹。在空气中50 kPa的二氧化碳条件下，35摄氏度时生长速率的相对抑制约为30%，30摄氏度时为50%，5摄氏度时为90%。因此，二氧化碳的抑制作用随温度降低而依次增强，这一效应可由二氧化碳溶解度随温度降低而增加来解释。蜡样芽孢杆菌在厌氧条件下（101 kPa氮气）的生长在40摄氏度时最佳，在低于18摄氏度和高于46摄氏度时停止。在最佳生长温度下101 kPa二氧化碳的相对抑制作用约为40%；在接近最大和最小生长温度时，这一比例增加到100%。温度降低（低于最佳温度）和二氧化碳对蜡样芽孢杆菌的生长抑制作用大于因二氧化碳在较低温度下溶解度增加所预期的抑制作用。
