人嗜碱性粒细胞脱颗粒的流式细胞术分析。II. 抗IgE、抗IgG4和钙离子载体A23187诱导的脱颗粒

Flow-cytometric analysis of human basophil degranulation. II. Degranulation induced by anti-IgE, anti-IgG4 and the calcium ionophore A23187.

作者信息

Nakagawa T, Stadler B M, Heiner D C, Skvaril F, de Weck A L

出版信息

Clin Allergy. 1981 Jan;11(1):21-30. doi: 10.1111/j.1365-2222.1981.tb01561.x.

DOI:10.1111/j.1365-2222.1981.tb01561.x

Abstract

Quantification of human basophil degranulation induced by anti-IgE, anti-IgG4, and by ionophore was performed using a flow-cytometric system. It was shown that these antibodies and ionophore can degranulate basophils in a dose-dependent manner, and that there is a wide variation in the response of basophils obtained from different individuals to these stimuli. A significant correlation was observed between the degree of degranulation induced by anti-IgE and anti-IgG4, while this was not the case for anti-IgE and ionophore. It was also shown that IgG4 myeloma protein can passively sensitive basophils. In general, degranulating efficacy was in the order of ionophore greater than anti-IgE greater than anti-IgG4, both in allergic and non-allergic individuals.

摘要

使用流式细胞术系统对由抗IgE、抗IgG4和离子载体诱导的人嗜碱性粒细胞脱颗粒进行定量分析。结果表明，这些抗体和离子载体能够以剂量依赖的方式使嗜碱性粒细胞脱颗粒，并且从不同个体获得的嗜碱性粒细胞对这些刺激的反应存在很大差异。观察到抗IgE和抗IgG4诱导的脱颗粒程度之间存在显著相关性，而抗IgE和离子载体之间则不存在这种情况。还表明IgG4骨髓瘤蛋白可使嗜碱性粒细胞被动致敏。总体而言，无论是在过敏个体还是非过敏个体中，脱颗粒效力的顺序为离子载体大于抗IgE大于抗IgG4。