Hörmann H, Jelinić V
Hoppe Seylers Z Physiol Chem. 1981 Jan;362(1):87-94. doi: 10.1515/bchm2.1981.362.1.87.
Trypsinized peritoneal macrophages of guinea pigs moderately bound soluble native 125I-collagen, type III. The association was slightly improved by plasma fibronectin. An additional considerable enhancement was achieved by heparin or heparan sulfate in presence of fibronectin. The enhancing effect of the two N-sulfated glycosaminoglycans was observed over a broad concentration range and, in case of heparin, exhibited a maximum near 1 mg/ml. Further rise of concentration resulted in a decline of binding. The related heparan sulfate potentiated binding less effectively than heparin. Optimal efficiency was recorded with lower concentrations. Hyaluronic acid, in higher concentrations, suppressed the enhancing effect of heparin or heparan sulfate on collagen binding. The improved attachment of native collagen, type III, to cells in presence of fibronectin and N-sulfated glycosaminoglycans was explained by a heparin/heparan sulfate induced conversion of soluble fibronectin to fibrils which showed an increased affinity to rod-like collagen molecules as well as to cell-surface structures. Hyaluronic acid, probably, inhibited the interaction of fibronectin fibrils with these substrates. Heparin and heparan sulfate, also in the absence of fibronectin, improved within a limited concentration range the binding of native 125I-collagen, type III, to the cells. The amount attached was, however, considerably less than in presence of fibronectin.
豚鼠经胰蛋白酶处理的腹腔巨噬细胞能适度结合可溶性天然III型125I标记胶原蛋白。血浆纤连蛋白可使这种结合略有改善。在纤连蛋白存在的情况下，肝素或硫酸乙酰肝素可进一步显著增强这种结合。在较宽的浓度范围内均观察到这两种N - 硫酸化糖胺聚糖的增强作用，对于肝素而言，在浓度接近1mg/ml时表现出最大值。浓度进一步升高会导致结合下降。相关的硫酸乙酰肝素增强结合的效果不如肝素有效，在较低浓度时记录到最佳效率。高浓度的透明质酸会抑制肝素或硫酸乙酰肝素对胶原蛋白结合的增强作用。在纤连蛋白和N - 硫酸化糖胺聚糖存在的情况下，III型天然胶原蛋白与细胞的附着改善，这是由于肝素/硫酸乙酰肝素诱导可溶性纤连蛋白转化为纤维，这些纤维对棒状胶原蛋白分子以及细胞表面结构表现出更高的亲和力。透明质酸可能抑制了纤连蛋白纤维与这些底物的相互作用。即使在没有纤连蛋白的情况下，肝素和硫酸乙酰肝素在有限的浓度范围内也能改善III型天然125I标记胶原蛋白与细胞的结合。然而，附着的量远少于存在纤连蛋白时的量。
