[Effect of 2 methods of demineralization on the on the preservation of glycoproteins and proteoglycans in the intertubular and peritubular dentin in the horse].

The effect of 2 methods of demineralization on the preservation of proteoglycans and glycoproteins was studied in the intertubular and peritubular dentine of the horse. The specimens embedded in Epon were demineralized with a 2% acid formic solution (Bonucci and Gheradi, 1975). Other fragments were treated with an organic solution of EDTA alkylammonium salt (Scott and Kyffin, 1979). These methods preserved in a satisfactory way these labile organic components. In the intertubular dentine, glycoproteins and proteoglycans were also identified, either associated with collagen fibres as a glue and granules, or present in the interfibrillar spaces. In the peritubular dentine, amorphous material formed a fine network between the mineral structures.