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Crystallization and preliminary X-ray investigation of human erythrocytic purine nucleoside phosphorylase.

作者信息

Cook W J, Ealick S E, Bugg C E, Stoeckler J D, Parks R E

出版信息

J Biol Chem. 1981 Apr 25;256(8):4079-80.

PMID:6783653
Abstract

Crystals of human erythrocytic purine nucleoside phosphorylase have been grown from solutions of ammonium sulfate. The crystals are trigonal, space group R32; the hexagonal axes are a = 143.8(2) and c = 165.1(2) A. The crystals are moderately stable to x-rays and diffract beyond 3.0 A resolution. The experimental density of the crystals indicates that the molecular weight of the protein is 94,000. The three subunits are not related by crystallographic symmetry.

摘要

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Purine nucleoside phosphorylase from human erythrocytes.
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Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization.
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